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lin28  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc lin28
    Lin28, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lin28/LIN28A+Rabbit+mAb/pmc12974335-132-73-74
    Average 93 stars, based on 30 article reviews
    lin28 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: xCT contributes to colorectal cancer tumorigenesis through upregulation of the MELK oncogene and activation of the AKT/mTOR cascade
    Article Snippet: Antibodies against xCT (ab37185, WB:1:1000, IF: 1:200), CD133 (ab216323, Flow Cyt: 1:100), MELK (ab273015, WB:1:1000, IF: 1:200), S6K (ab32529, WB:1:1000), P-S6K (ab131436, WB:1:1000), β-Actin (ab8226, WB:1:4000) were purchased from Abcam (Cambridge, UK). .. Antibodies against PCNA (#13110, WB:1:1000, IF: 1:400), Vimentin (#5741, WB:1:1000, IF: 1:200), N-cadherin (#13116, WB:1:1000, IF: 1:200, IHC: 1:200), CD133 (#64326, WB:1:1000, IF: 1:200, IHC: 1:200), E-cadherin (#3195, IHC: 1:400), Ki67 (#9027, IHC: 1:400), E2F1 (#3742, WB:1:1000), P-AKT (#4060, WB:1:1000, IHC: 1:100), C-myc (#18583, WB:1:1000), MMP1 (#54376, WB:1:1000), EPCAM (#93790, WB:1:1000), LIN28 (#3695, WB:1:1000), SOX2 (#3579, WB:1:1000), mTOR (#2983, WB:1:1000), P-mTOR (#5536, WB:1:1000), AKT (#4691, WB:1:1000), 4EBP1 (#9644, WB:1:1000), P-4EBP1 (#2855, WB:1:1000) were obtained from Cell Signaling Technology, Inc. (Beverly, MA). .. OCT3/4 (sc-365509, WB: 1:500) was purchased from Santa Cruz Biotechnology (CA, USA).

    Immunohistochemistry:

    Article Title: xCT contributes to colorectal cancer tumorigenesis through upregulation of the MELK oncogene and activation of the AKT/mTOR cascade
    Article Snippet: Antibodies against xCT (ab37185, WB:1:1000, IF: 1:200), CD133 (ab216323, Flow Cyt: 1:100), MELK (ab273015, WB:1:1000, IF: 1:200), S6K (ab32529, WB:1:1000), P-S6K (ab131436, WB:1:1000), β-Actin (ab8226, WB:1:4000) were purchased from Abcam (Cambridge, UK). .. Antibodies against PCNA (#13110, WB:1:1000, IF: 1:400), Vimentin (#5741, WB:1:1000, IF: 1:200), N-cadherin (#13116, WB:1:1000, IF: 1:200, IHC: 1:200), CD133 (#64326, WB:1:1000, IF: 1:200, IHC: 1:200), E-cadherin (#3195, IHC: 1:400), Ki67 (#9027, IHC: 1:400), E2F1 (#3742, WB:1:1000), P-AKT (#4060, WB:1:1000, IHC: 1:100), C-myc (#18583, WB:1:1000), MMP1 (#54376, WB:1:1000), EPCAM (#93790, WB:1:1000), LIN28 (#3695, WB:1:1000), SOX2 (#3579, WB:1:1000), mTOR (#2983, WB:1:1000), P-mTOR (#5536, WB:1:1000), AKT (#4691, WB:1:1000), 4EBP1 (#9644, WB:1:1000), P-4EBP1 (#2855, WB:1:1000) were obtained from Cell Signaling Technology, Inc. (Beverly, MA). .. OCT3/4 (sc-365509, WB: 1:500) was purchased from Santa Cruz Biotechnology (CA, USA).

    Article Title: PLAG1 fusions define a third subtype of CNS embryonal tumor with PLAG family gene alteration.
    Article Snippet: .. Immunohistochemistry was performed on FFPE tissue sections on a Ventana BenchMark ULTRA Immunostainer using the ultraView Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ, USA) and the following antibodies: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), oligodendrocyte lineage transcription factor 2 (OLIG2; clone EPR2673, rabbit monoclonal, 1:50 dilution, Abcam, Cambridge, UK), Synaptophysin (clone MRQ-40, rabbit monoclonal, 1:160 dilution, Cell Marque Corp., Rocklin, CA, USA), epithelial membrane antigen (EMA, GP1.4, mouse monoclonal, dilution 1:1000, Thermo Fisher Scientific), Ki-67 (clone MIB-1, mouse monoclonal, dilution 1:100, Dako Agilent), LIN28 (A177, rabbit polyclonal, 1:50, Cell Signaling, Danvers, MA, USA), S100 (Z0311, rabbit polyclonal, 1:2000, Dako Agilent), Desmin (clone D33, mouse monoclonal, dilution 1:50, Dako Agilent), and CD99 (CONFIRM anti-CD99, O13, mouse, monoclonal, Roche, Basel, Switzerland). .. Representative H&E-stained sections and immunohistochemical stains were digitally scanned on an Aperio slide scanner and histological and immunohistochemical features were reviewed and annotated using ImageScope software (Leica Biosystems).

    Article Title: Epithelial-Mesenchymal Transition Axis Lin 28, ARID1A, And ELF3 As A Novel Prognostic Triad in Invasive Ductal Breast Carcinoma
    Article Snippet: .. Four microns sections from the paraffin-embedded tissue blocks were processed for hormone receptors immunohistochemistry using monoclonal antibodies against the ki 67 index, er receptors, pr receptors, and her2 receptors (santa cruz, california, biotechnology) and 2nd antibodies. lin28, arid1a, and elf3 immunohistochemical staining was carried out using tma staining as follows:(12, 13) following a 1:50 dilution in signal stain antibody, lin28 (3978, cell signaling technology) was saved with the sections for a whole night at 4°c. after the sections had been treated with the antibodies, they were washed three times for five minutes individually with tbs-t before being exposed to an anti-rabbit biotin antibody (ls-d1, lsbio) diluted in (1:300) blocking solution for one hour at room temperature. the vectastain abc-ap reagent (ak-5000), the substrate kit (sk-5100), vector red alkaline phosphatase, and tbs-t stayed used to wash the sections three times for five minutes each before staining (14, 15). .. Four microns sections from the paraffin-embedded tissue blocks were processed for hormone receptors immunohistochemistry using monoclonal antibodies against the ki 67 index, er receptors, pr receptors, and her2 receptors (santa cruz, california, biotechnology) and 2nd antibodies.

    Article Title: PLAG1 fusions define a third subtype of CNS embryonal tumor with PLAG family gene alteration
    Article Snippet: .. Immunohistochemistry was performed on FFPE tissue sections on a Ventana BenchMark ULTRA Immunostainer using the ultraView Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ, USA) and the following antibodies: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), oligodendrocyte lineage transcription factor 2 (OLIG2; clone EPR2673, rabbit monoclonal, 1:50 dilution, Abcam, Cambridge, UK), Synaptophysin (clone MRQ-40, rabbit monoclonal, 1:160 dilution, Cell Marque Corp., Rocklin, CA, USA), epithelial membrane antigen (EMA, GP1.4, mouse monoclonal, dilution 1:1000, Thermo Fisher Scientific), Ki-67 (clone MIB-1, mouse monoclonal, dilution 1:100, Dako Agilent), LIN28 (A177, rabbit polyclonal, 1:50, Cell Signaling, Danvers, MA, USA), S100 (Z0311, rabbit polyclonal, 1:2000, Dako Agilent), Desmin (clone D33, mouse monoclonal, dilution 1:50, Dako Agilent), and CD99 (CONFIRM anti-CD99, O13, mouse, monoclonal, Roche, Basel, Switzerland). .. Representative H&E-stained sections and immunohistochemical stains were digitally scanned on an Aperio slide scanner and histological and immunohistochemical features were reviewed and annotated using ImageScope software (Leica Biosystems).

    Incubation:

    Article Title: Exosomal circ0000549 promotes MNNG-induced gastric cancer through miR-15b-5p/KIF1B
    Article Snippet: .. The membranes were blocked with 5% skimmed milk for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against N-cadherin (Cell Signaling Technology, Inc; cat. no. 13116S; dilution: 1:1,000), E-cadherin (Cell Signaling Technology, Inc; cat. no. 3195S; dilution: 1:1,000), PCNA (Cell Signaling Technology, Inc.; cat. no. 13110S; dilution: 1:1,000), Nanog (Cell Signaling Technology, Inc.; cat. no. 4903S; dilution: 1:1,000), OCT4 (Cell Signaling Technology, Inc; cat. no. 2750; dilution: 1:1,000), Lin28 (Cell Signaling Technology, Inc.; cat. no. 8706S; dilution: 1:1,000), SOX2 (Cell Signaling Technology, Inc; cat. no. 23064S; dilution: 1:1,000), Vimentin (Cell Signaling Technology, Inc.; cat. no. 5741S; dilution: 1:1,000), HSP70 (Cell Signaling Technology, Inc.; cat. no. 4872S; dilution: 1:1,000), Alix (Cell Signaling Technology, Inc.; cat. no. 92880S; dilution: 1:1,000), TSG101 (Proteintech, Inc; cat. no. 28283-1-AP; dilution: 1:1,000), Calnexin (Cell Signaling Technology, Inc.; cat. no. 2679S; dilution: 1:1,000), KIF1B (Abcam; cat. no. ab72108; dilution:1:1,500) and GAPDH (Bioworld Technology, Inc.; cat. no. BS65483M; dilution: 1:2,000). .. After washing with TBST containing 0.1% Tween-20, membranes were incubated with appropriate horseradish peroxidase-conjugated secondary antibodies (Signalway Antibody LLC; cat. no. L3012; dilution: 1:5,000).

    Article Title: Exosomal circ0000549 promotes MNNG‑induced gastric cancer through miR‑15b‑5p/KIF1B.
    Article Snippet: .. The membranes were blocked with 5% skimmed milk for 1 h at room temperature and incubated overnight at 4 ̊C with primary antibodies against N‐cadherin (cell Signaling Technology, Inc; cat. no. 13116S; dilution: 1:1,000), E‐cadherin (cell Signaling Technology, Inc; cat. no. 3195S; dilution: 1:1,000), PcNA (cell Signaling Technology, Inc.; cat. no. 13110S; dilution: 1:1,000), Nanog (cell Signaling Technology, Inc.; cat. no. 4903S; dilution: 1:1,000), OcT4 (cell Signaling Technology, Inc; cat. no. 2750; dilution: 1:1,000), Lin28 (cell Signaling Technology, Inc.; cat. no. 8706S; dilution: 1:1,000), SOX2 (cell Signaling Technology, Inc; cat. no. 23064S; dilu‐ tion: 1:1,000), Vimentin (cell Signaling Technology, Inc.; cat. no. 5741S; dilution: 1:1,000), HSP70 (cell Signaling Technology, Inc.; cat. no. 4872S; dilution: 1:1,000), Alix (cell Signaling Technology, Inc.; cat. no. 92880S; dilution: 1:1,000), TSG101 (Proteintech, Inc; cat. no. 28283‐1‐AP; dilu‐ tion: 1:1,000), calnexin (cell Signaling Technology, Inc.; cat. no. 2679S; dilution: 1:1,000), KIF1B (Abcam; cat. no. ab72108; dilution:1:1,500) and GAPdH (Bioworld Technology, Inc.; cat. no. BS65483M; dilution: 1:2,000). .. After washing with TBST containing 0.1% Tween‐20, membranes were incubated with appropriate horseradish peroxidase‐conjugated secondary antibodies (Signalway Antibody LLc; cat. no. L3012; dilution: 1:5,000).

    Formalin-fixed Paraffin-Embedded:

    Article Title: PLAG1 fusions define a third subtype of CNS embryonal tumor with PLAG family gene alteration.
    Article Snippet: .. Immunohistochemistry was performed on FFPE tissue sections on a Ventana BenchMark ULTRA Immunostainer using the ultraView Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ, USA) and the following antibodies: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), oligodendrocyte lineage transcription factor 2 (OLIG2; clone EPR2673, rabbit monoclonal, 1:50 dilution, Abcam, Cambridge, UK), Synaptophysin (clone MRQ-40, rabbit monoclonal, 1:160 dilution, Cell Marque Corp., Rocklin, CA, USA), epithelial membrane antigen (EMA, GP1.4, mouse monoclonal, dilution 1:1000, Thermo Fisher Scientific), Ki-67 (clone MIB-1, mouse monoclonal, dilution 1:100, Dako Agilent), LIN28 (A177, rabbit polyclonal, 1:50, Cell Signaling, Danvers, MA, USA), S100 (Z0311, rabbit polyclonal, 1:2000, Dako Agilent), Desmin (clone D33, mouse monoclonal, dilution 1:50, Dako Agilent), and CD99 (CONFIRM anti-CD99, O13, mouse, monoclonal, Roche, Basel, Switzerland). .. Representative H&E-stained sections and immunohistochemical stains were digitally scanned on an Aperio slide scanner and histological and immunohistochemical features were reviewed and annotated using ImageScope software (Leica Biosystems).

    Article Title: PLAG1 fusions define a third subtype of CNS embryonal tumor with PLAG family gene alteration
    Article Snippet: .. Immunohistochemistry was performed on FFPE tissue sections on a Ventana BenchMark ULTRA Immunostainer using the ultraView Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ, USA) and the following antibodies: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), oligodendrocyte lineage transcription factor 2 (OLIG2; clone EPR2673, rabbit monoclonal, 1:50 dilution, Abcam, Cambridge, UK), Synaptophysin (clone MRQ-40, rabbit monoclonal, 1:160 dilution, Cell Marque Corp., Rocklin, CA, USA), epithelial membrane antigen (EMA, GP1.4, mouse monoclonal, dilution 1:1000, Thermo Fisher Scientific), Ki-67 (clone MIB-1, mouse monoclonal, dilution 1:100, Dako Agilent), LIN28 (A177, rabbit polyclonal, 1:50, Cell Signaling, Danvers, MA, USA), S100 (Z0311, rabbit polyclonal, 1:2000, Dako Agilent), Desmin (clone D33, mouse monoclonal, dilution 1:50, Dako Agilent), and CD99 (CONFIRM anti-CD99, O13, mouse, monoclonal, Roche, Basel, Switzerland). .. Representative H&E-stained sections and immunohistochemical stains were digitally scanned on an Aperio slide scanner and histological and immunohistochemical features were reviewed and annotated using ImageScope software (Leica Biosystems).

    Membrane:

    Article Title: PLAG1 fusions define a third subtype of CNS embryonal tumor with PLAG family gene alteration.
    Article Snippet: .. Immunohistochemistry was performed on FFPE tissue sections on a Ventana BenchMark ULTRA Immunostainer using the ultraView Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ, USA) and the following antibodies: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), oligodendrocyte lineage transcription factor 2 (OLIG2; clone EPR2673, rabbit monoclonal, 1:50 dilution, Abcam, Cambridge, UK), Synaptophysin (clone MRQ-40, rabbit monoclonal, 1:160 dilution, Cell Marque Corp., Rocklin, CA, USA), epithelial membrane antigen (EMA, GP1.4, mouse monoclonal, dilution 1:1000, Thermo Fisher Scientific), Ki-67 (clone MIB-1, mouse monoclonal, dilution 1:100, Dako Agilent), LIN28 (A177, rabbit polyclonal, 1:50, Cell Signaling, Danvers, MA, USA), S100 (Z0311, rabbit polyclonal, 1:2000, Dako Agilent), Desmin (clone D33, mouse monoclonal, dilution 1:50, Dako Agilent), and CD99 (CONFIRM anti-CD99, O13, mouse, monoclonal, Roche, Basel, Switzerland). .. Representative H&E-stained sections and immunohistochemical stains were digitally scanned on an Aperio slide scanner and histological and immunohistochemical features were reviewed and annotated using ImageScope software (Leica Biosystems).

    Article Title: PLAG1 fusions define a third subtype of CNS embryonal tumor with PLAG family gene alteration
    Article Snippet: .. Immunohistochemistry was performed on FFPE tissue sections on a Ventana BenchMark ULTRA Immunostainer using the ultraView Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ, USA) and the following antibodies: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), oligodendrocyte lineage transcription factor 2 (OLIG2; clone EPR2673, rabbit monoclonal, 1:50 dilution, Abcam, Cambridge, UK), Synaptophysin (clone MRQ-40, rabbit monoclonal, 1:160 dilution, Cell Marque Corp., Rocklin, CA, USA), epithelial membrane antigen (EMA, GP1.4, mouse monoclonal, dilution 1:1000, Thermo Fisher Scientific), Ki-67 (clone MIB-1, mouse monoclonal, dilution 1:100, Dako Agilent), LIN28 (A177, rabbit polyclonal, 1:50, Cell Signaling, Danvers, MA, USA), S100 (Z0311, rabbit polyclonal, 1:2000, Dako Agilent), Desmin (clone D33, mouse monoclonal, dilution 1:50, Dako Agilent), and CD99 (CONFIRM anti-CD99, O13, mouse, monoclonal, Roche, Basel, Switzerland). .. Representative H&E-stained sections and immunohistochemical stains were digitally scanned on an Aperio slide scanner and histological and immunohistochemical features were reviewed and annotated using ImageScope software (Leica Biosystems).

    Bioprocessing:

    Article Title: Epithelial-Mesenchymal Transition Axis Lin 28, ARID1A, And ELF3 As A Novel Prognostic Triad in Invasive Ductal Breast Carcinoma
    Article Snippet: .. Four microns sections from the paraffin-embedded tissue blocks were processed for hormone receptors immunohistochemistry using monoclonal antibodies against the ki 67 index, er receptors, pr receptors, and her2 receptors (santa cruz, california, biotechnology) and 2nd antibodies. lin28, arid1a, and elf3 immunohistochemical staining was carried out using tma staining as follows:(12, 13) following a 1:50 dilution in signal stain antibody, lin28 (3978, cell signaling technology) was saved with the sections for a whole night at 4°c. after the sections had been treated with the antibodies, they were washed three times for five minutes individually with tbs-t before being exposed to an anti-rabbit biotin antibody (ls-d1, lsbio) diluted in (1:300) blocking solution for one hour at room temperature. the vectastain abc-ap reagent (ak-5000), the substrate kit (sk-5100), vector red alkaline phosphatase, and tbs-t stayed used to wash the sections three times for five minutes each before staining (14, 15). .. Four microns sections from the paraffin-embedded tissue blocks were processed for hormone receptors immunohistochemistry using monoclonal antibodies against the ki 67 index, er receptors, pr receptors, and her2 receptors (santa cruz, california, biotechnology) and 2nd antibodies.

    Immunohistochemical staining:

    Article Title: Epithelial-Mesenchymal Transition Axis Lin 28, ARID1A, And ELF3 As A Novel Prognostic Triad in Invasive Ductal Breast Carcinoma
    Article Snippet: .. Four microns sections from the paraffin-embedded tissue blocks were processed for hormone receptors immunohistochemistry using monoclonal antibodies against the ki 67 index, er receptors, pr receptors, and her2 receptors (santa cruz, california, biotechnology) and 2nd antibodies. lin28, arid1a, and elf3 immunohistochemical staining was carried out using tma staining as follows:(12, 13) following a 1:50 dilution in signal stain antibody, lin28 (3978, cell signaling technology) was saved with the sections for a whole night at 4°c. after the sections had been treated with the antibodies, they were washed three times for five minutes individually with tbs-t before being exposed to an anti-rabbit biotin antibody (ls-d1, lsbio) diluted in (1:300) blocking solution for one hour at room temperature. the vectastain abc-ap reagent (ak-5000), the substrate kit (sk-5100), vector red alkaline phosphatase, and tbs-t stayed used to wash the sections three times for five minutes each before staining (14, 15). .. Four microns sections from the paraffin-embedded tissue blocks were processed for hormone receptors immunohistochemistry using monoclonal antibodies against the ki 67 index, er receptors, pr receptors, and her2 receptors (santa cruz, california, biotechnology) and 2nd antibodies.

    Staining:

    Article Title: Epithelial-Mesenchymal Transition Axis Lin 28, ARID1A, And ELF3 As A Novel Prognostic Triad in Invasive Ductal Breast Carcinoma
    Article Snippet: .. Four microns sections from the paraffin-embedded tissue blocks were processed for hormone receptors immunohistochemistry using monoclonal antibodies against the ki 67 index, er receptors, pr receptors, and her2 receptors (santa cruz, california, biotechnology) and 2nd antibodies. lin28, arid1a, and elf3 immunohistochemical staining was carried out using tma staining as follows:(12, 13) following a 1:50 dilution in signal stain antibody, lin28 (3978, cell signaling technology) was saved with the sections for a whole night at 4°c. after the sections had been treated with the antibodies, they were washed three times for five minutes individually with tbs-t before being exposed to an anti-rabbit biotin antibody (ls-d1, lsbio) diluted in (1:300) blocking solution for one hour at room temperature. the vectastain abc-ap reagent (ak-5000), the substrate kit (sk-5100), vector red alkaline phosphatase, and tbs-t stayed used to wash the sections three times for five minutes each before staining (14, 15). .. Four microns sections from the paraffin-embedded tissue blocks were processed for hormone receptors immunohistochemistry using monoclonal antibodies against the ki 67 index, er receptors, pr receptors, and her2 receptors (santa cruz, california, biotechnology) and 2nd antibodies.

    Blocking Assay:

    Article Title: Epithelial-Mesenchymal Transition Axis Lin 28, ARID1A, And ELF3 As A Novel Prognostic Triad in Invasive Ductal Breast Carcinoma
    Article Snippet: .. Four microns sections from the paraffin-embedded tissue blocks were processed for hormone receptors immunohistochemistry using monoclonal antibodies against the ki 67 index, er receptors, pr receptors, and her2 receptors (santa cruz, california, biotechnology) and 2nd antibodies. lin28, arid1a, and elf3 immunohistochemical staining was carried out using tma staining as follows:(12, 13) following a 1:50 dilution in signal stain antibody, lin28 (3978, cell signaling technology) was saved with the sections for a whole night at 4°c. after the sections had been treated with the antibodies, they were washed three times for five minutes individually with tbs-t before being exposed to an anti-rabbit biotin antibody (ls-d1, lsbio) diluted in (1:300) blocking solution for one hour at room temperature. the vectastain abc-ap reagent (ak-5000), the substrate kit (sk-5100), vector red alkaline phosphatase, and tbs-t stayed used to wash the sections three times for five minutes each before staining (14, 15). .. Four microns sections from the paraffin-embedded tissue blocks were processed for hormone receptors immunohistochemistry using monoclonal antibodies against the ki 67 index, er receptors, pr receptors, and her2 receptors (santa cruz, california, biotechnology) and 2nd antibodies.



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    Image Search Results


    Aberrant LIN28B expression and DNA repair pathway are associate with MSE. (a) Integrated analysis combining scRNA‐seq data from ascites in a constructed preclinical OC model; scRNA‐seq data from MA in OC patients ( n = 13), and gene mutation profiles from MSE in cancer patients ( n = 442) to explore novel dual‐targeting therapeutic strategies (created at BioRender.com). (b) Venn diagram analysis of DEG targets between the preclinical OC model and OC patients (Nat Cancer. 2023). GO‐BP enrichment analysis for (c) SNV gene mutations and (d) CNV gene mutations in MSE from cancer patients. (e) Waterfall plot for top 45 SNV genes and relative pathways. Genotype Quality (GQ): Minimum threshold of 20 (99% confidence in the genotype call). Read Depth (DP): Site‐specific depth filters (DP ≥ 10 for high‐confidence calls, adjusted for cohort mean depth). lternate Allele Support: Minimum alternative allele reads (≥ 3) and fraction (AF ≥ 0.25 for heterozygous germline calls). opulation Frequency: Filtering against population databases (gnomAD allele frequency < 0.01 for rare variants, < 0.001 for ultra‐rare). (f) Heatmap of CNV genes in MSE from cancer patients ( n = 442). Quality Metrics: Segment‐wise log2 ratio standard deviation or confidence score (e.g., CNVkit quality score > 0.5). Size Threshold: Typically, > 1 kb for targeted sequencing and > 10–50 kb for whole‐genome sequencing to exclude small, noisy calls. Copy State Threshold: Log2 ratio thresholds define copy states (e.g., deletion: log2 ratio < −0.4, duplication: log2 ratio > 0.2).

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    Article Title: PARPi Combining Nanoparticle LIN28B siRNA for the Management of Malignant Ascites

    doi: 10.1002/advs.202510547

    Figure Lengend Snippet: Aberrant LIN28B expression and DNA repair pathway are associate with MSE. (a) Integrated analysis combining scRNA‐seq data from ascites in a constructed preclinical OC model; scRNA‐seq data from MA in OC patients ( n = 13), and gene mutation profiles from MSE in cancer patients ( n = 442) to explore novel dual‐targeting therapeutic strategies (created at BioRender.com). (b) Venn diagram analysis of DEG targets between the preclinical OC model and OC patients (Nat Cancer. 2023). GO‐BP enrichment analysis for (c) SNV gene mutations and (d) CNV gene mutations in MSE from cancer patients. (e) Waterfall plot for top 45 SNV genes and relative pathways. Genotype Quality (GQ): Minimum threshold of 20 (99% confidence in the genotype call). Read Depth (DP): Site‐specific depth filters (DP ≥ 10 for high‐confidence calls, adjusted for cohort mean depth). lternate Allele Support: Minimum alternative allele reads (≥ 3) and fraction (AF ≥ 0.25 for heterozygous germline calls). opulation Frequency: Filtering against population databases (gnomAD allele frequency < 0.01 for rare variants, < 0.001 for ultra‐rare). (f) Heatmap of CNV genes in MSE from cancer patients ( n = 442). Quality Metrics: Segment‐wise log2 ratio standard deviation or confidence score (e.g., CNVkit quality score > 0.5). Size Threshold: Typically, > 1 kb for targeted sequencing and > 10–50 kb for whole‐genome sequencing to exclude small, noisy calls. Copy State Threshold: Log2 ratio thresholds define copy states (e.g., deletion: log2 ratio < −0.4, duplication: log2 ratio > 0.2).

    Article Snippet: The following antibodies were used: anti‐human LIN28B (Cell Signaling Technology, 4196S, 1:1000), anti‐mouse Lin28b (Proteintech, 11724‐1‐AP, 1:500), anti‐β‐actin (ABclonal, AC026, 1:10000), StarBright Blue 520 goat anti‐mouse IgG (Bio‐Rad, 12005867, 1:10000), Alexa Fluor Plus 800 goat anti‐rabbit IgG (H + L) (Invitrogen, A32735, 1:10000), Anti‐Mouse CD8a‐Purified In vivo (C375‐25 mg), Anti‐Mouse NK1.1‐Purified In vivo (N123‐25 mg), Human/Primate IL‐6 Mab(Clone 6708, MAB206‐SP, R&D), Human TNF‐alpha Mab(Clone28401, MAB610‐SP, R&D), anti‐CD31 antibody (Thermo Fisher Scientific, MA3105, 1:200), and anti‐TER‐119 antibody (BD Biosciences, 557915, 1:100).

    Techniques: Expressing, Construct, Mutagenesis, Standard Deviation, Targeted Sequencing, Sequencing

    Synthesis and characterization of DSSP@lip‐PEG‐FA targeting LIN28B.(a) Schematic illustration of the synthesis process for siRNA/DSSP@lip‐PEG‐FA. Representative TEM images and size distribution profiles of siRNA/DSSP NPs (b) and siRNA/DSSP@lip‐PEG‐FA (c). (d) hydrogen peroxide (H 2 O 2 )‐ and glutathione (GSH)‐responsive siRNA release profiles under different conditions (pH 7.4, pH7.4& 1 mM H 2 O 2 , and pH 7.4 & 10 mM GSH) over 24 h. (e) Confocal microscopy images (scale bars: 50 µm) and quantitative analysis of cellular uptake efficiency for free siRNA, siRNA/DSSP@lip‐PEG, and siRNA/DSSP@lip‐PEG‐FA. (f) Flow cytometric analysis quantifying Cy3‐labeled siRNA fluorescence intensity in treated cells. (g) Flow cytometry evaluation of folate receptor (FA)‐targeting specificity. (h) Mean fluorescence intensity (MFI) comparison across different treatment groups at 6 h post‐incubation. (i) Confocal images showing intracellular distribution of siRNA/DSSP@lip‐PEG‐FA (scale bar: 10 µm). (j) Quantitative colocalization analysis performed using ImageJ software. (k) and (l) Western blot analysis of Lin28b protein expression in A2780 and ID8 ovarian cancer cells following various treatments. Data are presented as mean ± SD ( n = 3, unpaired t‐test). n = independent biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.

    Journal: Advanced Science

    Article Title: PARPi Combining Nanoparticle LIN28B siRNA for the Management of Malignant Ascites

    doi: 10.1002/advs.202510547

    Figure Lengend Snippet: Synthesis and characterization of DSSP@lip‐PEG‐FA targeting LIN28B.(a) Schematic illustration of the synthesis process for siRNA/DSSP@lip‐PEG‐FA. Representative TEM images and size distribution profiles of siRNA/DSSP NPs (b) and siRNA/DSSP@lip‐PEG‐FA (c). (d) hydrogen peroxide (H 2 O 2 )‐ and glutathione (GSH)‐responsive siRNA release profiles under different conditions (pH 7.4, pH7.4& 1 mM H 2 O 2 , and pH 7.4 & 10 mM GSH) over 24 h. (e) Confocal microscopy images (scale bars: 50 µm) and quantitative analysis of cellular uptake efficiency for free siRNA, siRNA/DSSP@lip‐PEG, and siRNA/DSSP@lip‐PEG‐FA. (f) Flow cytometric analysis quantifying Cy3‐labeled siRNA fluorescence intensity in treated cells. (g) Flow cytometry evaluation of folate receptor (FA)‐targeting specificity. (h) Mean fluorescence intensity (MFI) comparison across different treatment groups at 6 h post‐incubation. (i) Confocal images showing intracellular distribution of siRNA/DSSP@lip‐PEG‐FA (scale bar: 10 µm). (j) Quantitative colocalization analysis performed using ImageJ software. (k) and (l) Western blot analysis of Lin28b protein expression in A2780 and ID8 ovarian cancer cells following various treatments. Data are presented as mean ± SD ( n = 3, unpaired t‐test). n = independent biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.

    Article Snippet: The following antibodies were used: anti‐human LIN28B (Cell Signaling Technology, 4196S, 1:1000), anti‐mouse Lin28b (Proteintech, 11724‐1‐AP, 1:500), anti‐β‐actin (ABclonal, AC026, 1:10000), StarBright Blue 520 goat anti‐mouse IgG (Bio‐Rad, 12005867, 1:10000), Alexa Fluor Plus 800 goat anti‐rabbit IgG (H + L) (Invitrogen, A32735, 1:10000), Anti‐Mouse CD8a‐Purified In vivo (C375‐25 mg), Anti‐Mouse NK1.1‐Purified In vivo (N123‐25 mg), Human/Primate IL‐6 Mab(Clone 6708, MAB206‐SP, R&D), Human TNF‐alpha Mab(Clone28401, MAB610‐SP, R&D), anti‐CD31 antibody (Thermo Fisher Scientific, MA3105, 1:200), and anti‐TER‐119 antibody (BD Biosciences, 557915, 1:100).

    Techniques: Confocal Microscopy, Labeling, Fluorescence, Flow Cytometry, Comparison, Incubation, Software, Western Blot, Expressing